Generator
GmR

Part:BBa_P1012:Design

Designed by: Tom Knight   Group: Part Editors   (2006-03-15)


gentamycin/kanamycin resistance cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

primers to make Biobrick version of GmR gene cassette (P1012)

gtttcttcgaattcgcggccgcttctagagTGGACTTGACTGAGTTTATGGAA, P3aph-F

gtttcttcctgcagcggccgctactagtaTCTGGACTTGACTCACTTCCTTT, P3aph-R

P3aph-R (3' - 5')
aaaggaagtgagtcaagtccagatactagtagcggccgctgcaggaagaaac



Source

Originally from Tn4001 transposon; sequence M18086. Created from plasmid pISM2062 from Chris Minion, Iowa State University.

References